Organotypic brain slice models are vital tools in neuroscience research, providing a three-dimensional, in vitro system that closely mimics the in vivo brain environment. These models maintain the architecture and connectivity of brain tissue, enabling the study of neural networks, synaptic functions, and disease mechanisms. They are instrumental in researching neurological disorders such as epilepsy, Alzheimer’s, and Parkinson’s disease, facilitating drug testing and advancing our understanding of brain physiology.